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Chimie moléculaire Paris Centre
ÉCOLE DOCTORALE 406

The power of chemoselectivity: functional protein-conjugates for intra- and extracellular targeting

Séminaire du Dr Christian P. R. Hackenberger (Humboldt University of Berlin)

Abstract :

Our lab aims to identify new bioorthogonal reactions for the synthesis and modification of functional peptides and proteins. We apply these highly selective organic reactions1 to study functional consequences of naturally occurring posttranslational protein modifications (PTMs), in particular phosphorylated Lys- and Cystein-peptides,2 as well as to generate novel peptide- and protein-conjugates for pharmaceutical and medicinal applications. In this presentation l will focus on the chemical modification of functional proteins as well as their cellular delivery. Thereby, we employ cyclic cell penetrating peptides (cCPPs) to transport a functional full length protein to specific intracellular compartments of living cells as recently demonstrated by the direct delivery of different fluorescent proteins.3 For protein modification we use a combined approach of intein expression as well as recently developed chemoselective reactionsand enzymatic ligations, for instance the so-called P54 or Tub-tag labeling.5  This concept is finally applied to generate new antibody-drug conjugates4b as well as cell-permeable nanobodies, i.e. small antigen binding proteins that remain active within the reductive milieu inside living cells, to interfere with intracellular targets.6

02/03/20

Traductions :

    Contact

    Directeur

    Dr Cyril OLLIVIER (cyril.ollivier @ upmc.fr)

     

    Directeur adjoint PSL

    Dr Fethi BEDIOUI (fethi.bedioui @ chimieparistech.psl.eu)

     

    Secrétariat

    Irène RASOARINORO  (irene.rasoarinoro @ upmc.fr)

    Tel. : 01 44 27 38 48

     

    SU - ED 406

    Tour 32 - 42

    Bureau 114 (1er étage)

    BC 63 - 4 Place Jussieu 

    75252 Paris cedex 5