From antibodies to MOFs: a full set of envelopes for metal cofactors in order to build up new artificial metalloenzymes

Séminaire du Pr Jean-Pierre Mahy (Paris-Saclay University)

Abstract :

. Our group has long been involved in the production of artificial Metalloenzymes that have been found able to catalyze stereo-selective oxidation of chemicals by H₂O₂, RNAse like hydrolysis of oligonucleotides and stereoselective Diels-Alder reactions. The most recent developments of our research will be presented here:

- The design of new artificial metalloenzymes that catalyze the stereoselective Diels-Alder reaction “in vivo”. The use of such biocatalysts for theranostic purposes will be introduced.

- The elaboration of totally artificial metalloenzymes by:

(i) insertion of metal complexes into a new family of artificial proteins based on a thermostable alpha-helical repeated motif (αRep),

(ii) insertion of a minienzyme, microperoxidase, into Metal Organic Frameworks (MOF),

iii) association of polyoxometallates (POMs) with artificial polyimine polymer-based

reductases, to afford ArMs that catalyse the oxidation of organic substrates by O₂ in water.

10/01/20

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